"Grid Screen" crystallization kit
pathology at a1.mscf.upenn.edu
Fri Jan 8 14:14:18 EST 1993
Below are the responses that I received to my inquiry about the
usefulness of "Grid Screen" crystallization kits from Hampton Research.
Thanks to everyone who responded.
Department of Pathology
University of Pennsylvania
email: pathology at a1.mscf.upenn.edu
Subject: Crystqallization screens
The Hampton Research crystallization screens are very good! If you are
starting your search for initial crystallization conditions, I recommend
the Crystal Screen kit. I've had terrific results with it! It contains
50 different combinations of salts, alcohols, PEGs, etc---& is one of the
best screens around. I have not used the AS or PEG Grid Screens as much
(they haven't been on the market very long), but did use them at the Cold
Spring Harbor Lab. Macromolecular Crystallography course this Oct. with
good results. So I would also recommend them too. Bob Cudney at
Hampton Res. is extremely helpful----so if have any questions, call him.
Or if I can help--
e mail me at amh12478 at usav01.glaxo.com
Glaxo Research Institute
Subject: Xtal Screen Kits
Regarding your questions about the Hampton xtal screening kits: I'm a
cloner in a structure function lab, but I can tell you (a) our
xtallographers us'em and (b) they love them - great time savers. The vast
majority of your boss's expenses are probably salary and benefits, and
these kits apparently save enormous amounts of time. Big productivity
improvement. That's about the limit of my knowledge. For more info,
contact one of our xtal people by email.
Try batalia at utbc01.cm.utexas.edu - he'll be here over the holidays.
READY at UTBC01.CM.UTEXAS.EDU |
Clayton Foundation Biochemical Institute
Subject: RE: "Grid Screen" Crystallization Kit
not cheap but it does work. good luck.
epr at leland.Stanford.EDU
Subject: Grid screen
This is about your query on bionet.xtallography about the grid screen
from Hampton. It is my personal experience that it is extremely helpful.
Since it is very representative of the conditions under which the
proteins have bee crystallized so far, the probability of hitting
conditions close to optimum for a new protein arisgood. So when working
on a new protein I would definitely use the grid screen to begin with.
However, I would also set up plates with ammonium sulfate, PEG (400,
3350, 8000). By the way, I am a graduate student in the Dept. of Biochem
and Biophy at the Texas A&M University.
n053gf at tamuts.tamu.edu
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