DNA-Protein cocrystallization

WILLARD at WISTB.WISTAR.UPENN.EDU WILLARD at WISTB.WISTAR.UPENN.EDU
Tue Mar 9 13:44:00 EST 1993


Does anyone know of ways to solubilize protein-DNA complexes?  In several
attempts to form oligonucleotide (20-21mers) Protein complexes, I have found
that a precipitate forms either immediately or shortly after mixing the two
components.  I have tried Mg++, spermine, glycerol, and NaCl.  Any suggestions
would be greatly appreciated.

My E mail address is Willard at wistb.wistar.upenn.edu.

Thanks,
Rob Willard.




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