Proline cis/trans isomerisation

[Ben Davis]

In article <1993Oct27.135711.17403 at infodev.cam.ac.uk>, smb18 at mbfs.bio.cam.ac.uk (Simon Brocklehurst) writes:
|> bjd12 at cus.cam.ac.uk (Ben Davis) writes:
|> 
|>	<stuff deleleted>

|> >	(b) how far (typically) does the cis:trans isomerisation affect the
|> >chemical shifts observed in other residues
|> >	
|>       Which nuclei do you mean???
	
	1H, 15N. I see what appear to be changes of around 0.25ppm for Ca protons
between two forms, for residues (trp, lys) close to different Pro; this seems
large to me.

|> 
|> 
|>    As an aside, could I ask how many, if any, disulphides your peptide has?
	
	None; its a 40mer cleaved from a larger protein (barley CI2) with CNBr	
|> 
|> 
|> !-----------------------------------------------------------------------
|> Simon M. Brocklehurst
|> Cambridge Centre for Molecular Recognition
|> Department of Biochemistry
|> University of Cambridge
|> Cambridge
|> UK
|> 
|> E-mail: smb18 at mbfs.bio.cam.ac.uk