Does anyone know of a way to run IEF gels of DNA binding proteins, specifically
Zinc finger proteins? I have attempted to run an IEF gel for the purpose
of assessing the ion exchange purification conditions of two different zinc
finger proteins - WT1 (four Zn fingers) and Mig1 (two zinc fingers). I used
the Phast system from pharmacia, including their pre-cast IEF 3-9 gels.
Using silver staining, I could see that the proteins PRECIPITATED at the
point of application (no matter where they were applied on the gel).
This has been a major problem because I cannot determine whether or not
my sample is homogeneous - the possibility exists that there are multiple
Any suggestions would be greatly appreciated.
Wistar Institute/Univ of Penn
Willard at WISTB.WISTAR.UPENN.EDU