What's the largest prot. structure known?

Gerard Kleijwegt gerard at rigel.bmc.uu.se
Fri Aug 19 13:27:47 EST 1994


In article <3311hh$jj9 at columba.udac.uu.se>, gerard at rigel.bmc.uu.se (Gerard Kleijwegt) writes:
|> In article <zjons-180894211142 at 130.60.120.10>, zjons at vetbio.unizh.ch (Zophonias O. Jonsson) writes:
|> |> Dear Xtallographers
|> |> 
|> |>   I am a layman who just loves to see those beautiful structures they show
|> |> in full color in Nature and Science.  Last year I was fortunate enough to
|> |> get to attend some lectures of men with names in the protein structure
|> |> community.  If I remember correctly one of them (Alwin Jones I think) said
|> |> that the lagrest structure determined to a reasonable (>3a) resolution was
|> |> somewhre close to 60 KDa.  Now I have three questions for you:
|> |> 
|> |> 1) Does my memory serve me right?  (Given the fact that those guys would
|> |> not have said anything that wasn't true)
|> 
|> i'm sure it does
|> 
|> |> 2) What is the current record (the largest single peptide that has had its
|> |> diffraction pattern traced)?
|> 
|> the largest i could find in a five-minute quick-n-dirty search
|> is 842 amino acids, of which 833 could be traced (~6800 non-H atoms;
|> assuming 14 grams/mole of atoms (to account for hydrogens & S) -> ~95 kDa)
|> this is glycogen phosphorylase (PDB id 3GPB), solved at 2.3 A
|> 
|> some multi-protein complexes are bigger than this (e.g. rubisco
|> L8H8, photosynthetic reaction centre) but they don't consist
|> of a single polypeptide
|> 
|> (and of course virus coats are larger, but they don't consist of
|> a single polypeptide chain but rather, for instance, of 60 copies
|> each of three smaller chains of ~130 residues, i.e. total of
|> 60 * 3 * 130 residues)
|> 

well, protein xtallographers travel faster than light
found out today that brian matthews' group has solved
a single chain of 1023 (almost 2^10 ..) residues
was in nature recently

--gerard




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