clarsen at bimcore.emory.edu
Tue Aug 30 09:00:56 EST 1994
Would it be worth my time to attempt crystallization of a 25kDa enzyme and its
8kDa substrate if the two were covalently bound?
The binding constant is 0.5 uM.
Each protein can be crystallized independently.
The larger protein crystals do not stabilize as a mercury derivative, with three cysteines
The smaller does not have any cys and stays native at 50% methanol.
Would there be too much flexibility at the junction?
Chris (I have all the tools and experience to do this immediately) Larsen
(Searching for a Post doc in Biochemistry)
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