cryo crystallography

Lori Kohlstaedt kohlstaedt at sbmm1.ucsb.edu
Fri Nov 4 01:34:55 EST 1994


David Jeruzalmi, a grad student in Tom Steitz' group has developed a method
of making loops from opthalomolgists' suture for freezing crystals.  This
stuff is much thinner that other materials used for loops, resulting in less
eclipsing of the diffraction pattern.  We used David's loop making method
when I worked out freezing conditions for our C2 reverse transcriptase
crystals.  It was nice to not surrender much of a wedge of data to the loop
with such low symmetry.  David has made a nice set of directions for making
loops.  I think he wouldn't mind sharing them.  His e-mail address is
jeruzami at hhvms8.csb.yale.edu.
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Lori Kohlstaedt
Dept. of Chemistry, UCSB
(805) 893-8687
<Reciprocal Space, the Final Frontier>
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