cryo crystallography

Agustin de la Calle agustin at GREEN.DFCI.HARVARD.EDU
Fri Nov 4 07:30:24 EST 1994


Lori,
Do you know the price range of the surgical stitch? I guess if you can 
use just plain ol' dental floss you couldn't beat this price. Of course 
one has first to check if this brand you'd be using causes disturbing 
diffraction (since it's supposedly a material with an ordered structure). 
Much better (and even cheaper) worked a loop made out of a hair. It's 
not so thin and flimsy and _I_ could form the loop much easier. But a 
double loop in case your crystal would be rather spherical could be quite 
a challenge. In any case one should first put the loop alone in the beam 
and test the diffractive properties to be sure it wouldn't interfere with 
the real measurement.
Good luck,
	Agustin
==============================================================================
	Agustin de la Calle    		|
	X-ray Crystallography		|	voice:(617) 632-4754
	Dana-Farber Cancer Institute	|	fax:  (617) 632-4393
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On Fri, 4 Nov 1994, Lori Kohlstaedt wrote:

> David Jeruzalmi, a grad student in Tom Steitz' group has developed a method
> of making loops from opthalomolgists' suture for freezing crystals.  This
> stuff is much thinner that other materials used for loops, resulting in less
> eclipsing of the diffraction pattern.  We used David's loop making method
> when I worked out freezing conditions for our C2 reverse transcriptase
> crystals.  It was nice to not surrender much of a wedge of data to the loop
> with such low symmetry.  David has made a nice set of directions for making
> loops.  I think he wouldn't mind sharing them.  His e-mail address is
> jeruzami at hhvms8.csb.yale.edu.
> ***************************************
> Lori Kohlstaedt
> Dept. of Chemistry, UCSB
> (805) 893-8687
> <Reciprocal Space, the Final Frontier>
> ***************************************
> 
> 




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