His tagged proteins

C.S.RAMAN raman at BIOC01.UTHSCSA.EDU
Thu Apr 20 09:10:12 EST 1995


Duncan:

> Has anyone got a clue as to the structure of the histidine tag sometimes 
> used in modified proteins for ease of purification.
> I want to do some modeling in the n-terminus of a protein with a 
> (his)6-tag.
> 

To my knowledge there is only one protein whose structure has been
solved with the his-tag.  It is the map kinase structure from Betsy
Goldsmith's laboratory (UTSW, Dallas).  You might want to look at the
following references:


ZHANG FM; ROBBINS DJ; COBB MH; GOLDSMITH EJ.
     CRYSTALLIZATION AND PRELIMINARY X-RAY STUDIES OF EXTRACELLULAR
   SIGNAL-REGULATED KINASE-2/MAP KINASE WITH AN INCORPORATED HIS-TAG.
     JOURNAL OF MOLECULAR BIOLOGY, 1993 OCT 5, V233 N3:550-552.


ZHANG FM; STRAND A; ROBBINS D; COBB MH; and others.
     ATOMIC STRUCTURE OF THE MAP KINASE ERK2 AT 2.3-ANGSTROM RESOLUTION.
     NATURE, 1994 FEB 24, V367 N6465:704-711.


Cheers
-raman
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