Hi,
We have been growing protein crystals in
hanging drops, at various temperatures (ie. 4, 10
16, 21 C) from either salt or PEG solutions, but
they are invariably hollow in nature. It seems that
this is most likely due to the crystals growing too
fast once nucleation has taken place. I have read
that poisoning the mother liquor (with detergent,
etc) can sometmes help. Does anyone have any other
suggestions based on experience with this problem ?
Thank-you
Allan Matte
Dept. of Biochemistry
University of Saskatchewan
matte at skyfox.usask.ca
