Requests for Beamtime at NSLS beamline X8C

STEVE 282-3018, 516 GINELL at BNLVS2.NSLS.BNL.GOV
Wed Jan 11 10:59:56 EST 1995


 			 THE STRUCTURAL BIOLOGY CENTER
                           Argonne National Laboratory
        	           9700 South Cass Avenue
                           Argonne, Illinois  60439	     	
								
							

            We are now soliciting proposals to use our experimental station
       at beamline X8C of the National Synchrotron Light Source (NSLS). Please
       see the 1995 calendar for deadlines.  Your proposal should be brief
       (see example format), consisting of a page for crystallographic data
       and a page of scientific discussion.  Each proposal is reviewed and
       rated, according to the following criteria:

          Scientific Significance: 		                  40%
          Need for Synchrotron Source (difficulty):               25%
          Feasibility (likelihood of success):                    25%
          Degree of Commitment
              (manpower, funding, effort expended by proposer):   10%

            Beamline X8C is a monochromatic, focussed x-ray source, tunable
       over an energy range of 6 to 20 keV (Lambda = 2.0 to 0.6 A), giving us
       access to the absorption edges of most heavy atoms useful for M.I.R.
       phasing.  Its energy resolution (about 3 eV) is also excellent for
       multiple-energy anomalous dispersion (MAD) phasing. The beamline has
       high spectral brightness.  It is about 600 times more intense than a 
       Rigaku RU200, with 50-fold better spectral purity and 25-fold better
       spatial focus relative to the rotating anode source.

            At X8C we operate an electronic area detector with a 1,024 x 1,024 
       pixel CCD sensor, having a circular active area 114 mm in diameter; its
       100 um pixels are commensurate with its point-spread function size.
       This detector has a dynamic range exceeding 10,000:1 and a 50%
       efficiency, making it an extraordinarily useful data collection tool.
       During the winter of 1995 we will replace it with an even more advanced,
       3,072 x 3,072 pixel detector, now being tested.

            The detector is mounted on a Huber 4-circle goniostat under 
       computer control.  Experimenters may choose to cool their crystals with
       an FTS air refrigerator, or to flash-cool their crystals with a MSC 
       liquid nitrogen cooling apparatus.  The beamline and CCD detector
       are controlled by a VAX 4000/600. Data processing is performed on four 
       Silicon Graphics Iris Indigos and two VAXstation 4000/90 workstations
       with approximately 20 Gbytes of conventional disk space. Data can be 
       archived using several different types of tape drives.  The computer 
       system is accessible through e-mail. Data collection and processing 
       are carried out by MADNES, which is supported by James Pflugrath at 
       Molecular Structure Corp.  We have established a small biochemistry
       laboratory for crystal growth, derivative soaks, and crystal mounting 
       and prealignment. During the spring of 1995 we will be moving into 
       the new NSLS biology wing with laboratory and cold room facilities 
       directly adjacent to the X8C beamline. User offices are also available.

            This project is currently supported by the Department of Energy,
       Office of Health and Environmental Research.

            To submit your proposals, you may use e-mail, FAX, or conventional
       mail.  We prefer proposals be sent in a machine-readable format.  Send
       your proposals to:

                      Aine O'Carroll, SBC Admin. Ass't., Attn: X8C Program
                      Argonne National Laboratory, Bldg. 202
                      9700 South Cass Avenue
                      Argonne, Illinois 60439
                      (708) 252-5335
                      (708) 252-6126 FAX
                      OCARROLL at ANBIW4.SBC.ANL.GOV



       Edwin M. Westbrook, Director          Stephan L. Ginell, Coordinator
       Structural Biology Center       	     X8C SBC User Facility
       Phone: (708) 252-3983                 Phone: (516) 282-3018
       EMAIL: WESTBROOK at ANL.GOV              EMAIL: GINELL at BNL.GOV


--------------------------------------------------------------------------------



 	NSLS Beamline X8C 1995 Calendar 

 31 January 1995   New proposals for the 1 May-30 August 1995 trimester must
		      be received in the SBC office at Argonne National Labs
  1 April 1995     Users will be notified of their scheduled time for the
	              1 May-30 August 1995 cycle
 31 May 1995       New proposals for the 1 September-31 December 1995
		      trimester must be received in the SBC office at Argonne
	 	      National Labs
  1 August 1995    Users will be notified of their scheduled time for the 	
		      1 September-31 December 1995 cycle
 30 September 1995 New proposals for the 1 January-30 April 1995 trimester must
		      be received in the SBC office at Argonne National Labs
  1 December 1995  Users will be notified of their scheduled time for the
        	      1 January-30 April 1995 cycle
 
	Submitted proposals will remain in the eligible pool for one year.

------------------------------------------------------------------------------

                      PROPOSAL FOR X8C BEAMLINE TIME ALLOCATION


      INVESTIGATOR INFORMATION                      PROJECT INFORMATION

PRINCIPAL INVESTIGATOR __________________   NAME OF PROJECT __________________
INSTITUTION       _______________________   FULL NAME OF MOLECULE ____________  
ADDRESS(Department) _____________________      UNIT CELL PARAMETERS   
(Street)         ________________________           a=____  	alpha=____     
(City,State,Zip) ________________________           b=____      beta=____
PHONE NUMBER  ___________________________           c=____      gamma=____
FAX NUMBER   ____________________________
E-MAIL ADDRESS   ________________________
FUNDING SOURCE    _______________________    SPACE GROUP(Int.Tables) __________
OTHER INVESTIGATORS _____________________    POLYMERS PER A.U.     __________
(Please List All)  ______________________    Vm   _______________   _____
_________________________________________    CRYSTAL SIZE (mm)  _________
_________________________________________
_________________________________________
_________________________________________

                 TYPE OF MOLECULE (Please circle or mark one):

PROTEIN -unspecified    VIRUS -unspecified    DNA -all     RNA -unspecified  
        -enzyme               -plant             types         -enzyme         
        -DNA binding          -animal                          -transfer      
        -RNA binding          -human                           -ribosomal  
        -toxin                                                 -messenger
        -complex 
  
 COMPLEX -unspecified         COMPLEX -protein-ligand
         -multiprotein                -DNA-ligand
         -protein-DNA                 -RNA-ligan
         -protein-RNA
         -ribosome
         -virus-drug
  

                              PROJECT HISTORY

PREV SYNCHROTRON DATA  COLLECTION?  YES/NO (If NO, skip next 4 lines)  
   SYNCHROTRON FACILITY ID      _______________________________
   SYNCHROTRON DATA RESOLUTION  _______________________________
   SYNCHROTRON DATA RSYMM       _______________________________
   SYNCHROTRON DETECTOR         _______________________________

PREV LAB DATA  COLLECTION?  YES/NO (If NO, skip next 5 lines)
   LAB X-RAY SOURCE (Circle)  -Rotating Anode   -Fixed Target
   LAB X-RAY POWER (KW) _________________________________
   LAB DATA RESOLUTION  _______________________________
   LAB DATA RSYMM       _______________________________
   LAB X-RAY DETECTOR   _________________________________
         
                                                                         
                             PROPOSAL INFORMATION

PROPOSED DATA COLLECTION METHOD:    MIR    MAD     Mol.Repl.  Other___________  
PROPOSED DATA RESOLUTION          ______________     
SPECIAL REQUIREMENTS (Circle all that apply):  
            -Cold Air  ____deg C                  -Flash Freezing      
            -Chemistry Lab                        -Cold Room
            -On-site crystallizations             -On-site Derivative Soaks
            -Fluorescece detector              
            -Other _______________________




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