Requests for Beamtime at NSLS beamline X8C
STEVE 282-3018, 516
GINELL at BNLVS2.NSLS.BNL.GOV
Wed Jan 11 10:59:56 EST 1995
THE STRUCTURAL BIOLOGY CENTER
Argonne National Laboratory
9700 South Cass Avenue
Argonne, Illinois 60439
We are now soliciting proposals to use our experimental station
at beamline X8C of the National Synchrotron Light Source (NSLS). Please
see the 1995 calendar for deadlines. Your proposal should be brief
(see example format), consisting of a page for crystallographic data
and a page of scientific discussion. Each proposal is reviewed and
rated, according to the following criteria:
Scientific Significance: 40%
Need for Synchrotron Source (difficulty): 25%
Feasibility (likelihood of success): 25%
Degree of Commitment
(manpower, funding, effort expended by proposer): 10%
Beamline X8C is a monochromatic, focussed x-ray source, tunable
over an energy range of 6 to 20 keV (Lambda = 2.0 to 0.6 A), giving us
access to the absorption edges of most heavy atoms useful for M.I.R.
phasing. Its energy resolution (about 3 eV) is also excellent for
multiple-energy anomalous dispersion (MAD) phasing. The beamline has
high spectral brightness. It is about 600 times more intense than a
Rigaku RU200, with 50-fold better spectral purity and 25-fold better
spatial focus relative to the rotating anode source.
At X8C we operate an electronic area detector with a 1,024 x 1,024
pixel CCD sensor, having a circular active area 114 mm in diameter; its
100 um pixels are commensurate with its point-spread function size.
This detector has a dynamic range exceeding 10,000:1 and a 50%
efficiency, making it an extraordinarily useful data collection tool.
During the winter of 1995 we will replace it with an even more advanced,
3,072 x 3,072 pixel detector, now being tested.
The detector is mounted on a Huber 4-circle goniostat under
computer control. Experimenters may choose to cool their crystals with
an FTS air refrigerator, or to flash-cool their crystals with a MSC
liquid nitrogen cooling apparatus. The beamline and CCD detector
are controlled by a VAX 4000/600. Data processing is performed on four
Silicon Graphics Iris Indigos and two VAXstation 4000/90 workstations
with approximately 20 Gbytes of conventional disk space. Data can be
archived using several different types of tape drives. The computer
system is accessible through e-mail. Data collection and processing
are carried out by MADNES, which is supported by James Pflugrath at
Molecular Structure Corp. We have established a small biochemistry
laboratory for crystal growth, derivative soaks, and crystal mounting
and prealignment. During the spring of 1995 we will be moving into
the new NSLS biology wing with laboratory and cold room facilities
directly adjacent to the X8C beamline. User offices are also available.
This project is currently supported by the Department of Energy,
Office of Health and Environmental Research.
To submit your proposals, you may use e-mail, FAX, or conventional
mail. We prefer proposals be sent in a machine-readable format. Send
your proposals to:
Aine O'Carroll, SBC Admin. Ass't., Attn: X8C Program
Argonne National Laboratory, Bldg. 202
9700 South Cass Avenue
Argonne, Illinois 60439
(708) 252-5335
(708) 252-6126 FAX
OCARROLL at ANBIW4.SBC.ANL.GOV
Edwin M. Westbrook, Director Stephan L. Ginell, Coordinator
Structural Biology Center X8C SBC User Facility
Phone: (708) 252-3983 Phone: (516) 282-3018
EMAIL: WESTBROOK at ANL.GOV EMAIL: GINELL at BNL.GOV
--------------------------------------------------------------------------------
NSLS Beamline X8C 1995 Calendar
31 January 1995 New proposals for the 1 May-30 August 1995 trimester must
be received in the SBC office at Argonne National Labs
1 April 1995 Users will be notified of their scheduled time for the
1 May-30 August 1995 cycle
31 May 1995 New proposals for the 1 September-31 December 1995
trimester must be received in the SBC office at Argonne
National Labs
1 August 1995 Users will be notified of their scheduled time for the
1 September-31 December 1995 cycle
30 September 1995 New proposals for the 1 January-30 April 1995 trimester must
be received in the SBC office at Argonne National Labs
1 December 1995 Users will be notified of their scheduled time for the
1 January-30 April 1995 cycle
Submitted proposals will remain in the eligible pool for one year.
------------------------------------------------------------------------------
PROPOSAL FOR X8C BEAMLINE TIME ALLOCATION
INVESTIGATOR INFORMATION PROJECT INFORMATION
PRINCIPAL INVESTIGATOR __________________ NAME OF PROJECT __________________
INSTITUTION _______________________ FULL NAME OF MOLECULE ____________
ADDRESS(Department) _____________________ UNIT CELL PARAMETERS
(Street) ________________________ a=____ alpha=____
(City,State,Zip) ________________________ b=____ beta=____
PHONE NUMBER ___________________________ c=____ gamma=____
FAX NUMBER ____________________________
E-MAIL ADDRESS ________________________
FUNDING SOURCE _______________________ SPACE GROUP(Int.Tables) __________
OTHER INVESTIGATORS _____________________ POLYMERS PER A.U. __________
(Please List All) ______________________ Vm _______________ _____
_________________________________________ CRYSTAL SIZE (mm) _________
_________________________________________
_________________________________________
_________________________________________
TYPE OF MOLECULE (Please circle or mark one):
PROTEIN -unspecified VIRUS -unspecified DNA -all RNA -unspecified
-enzyme -plant types -enzyme
-DNA binding -animal -transfer
-RNA binding -human -ribosomal
-toxin -messenger
-complex
COMPLEX -unspecified COMPLEX -protein-ligand
-multiprotein -DNA-ligand
-protein-DNA -RNA-ligan
-protein-RNA
-ribosome
-virus-drug
PROJECT HISTORY
PREV SYNCHROTRON DATA COLLECTION? YES/NO (If NO, skip next 4 lines)
SYNCHROTRON FACILITY ID _______________________________
SYNCHROTRON DATA RESOLUTION _______________________________
SYNCHROTRON DATA RSYMM _______________________________
SYNCHROTRON DETECTOR _______________________________
PREV LAB DATA COLLECTION? YES/NO (If NO, skip next 5 lines)
LAB X-RAY SOURCE (Circle) -Rotating Anode -Fixed Target
LAB X-RAY POWER (KW) _________________________________
LAB DATA RESOLUTION _______________________________
LAB DATA RSYMM _______________________________
LAB X-RAY DETECTOR _________________________________
PROPOSAL INFORMATION
PROPOSED DATA COLLECTION METHOD: MIR MAD Mol.Repl. Other___________
PROPOSED DATA RESOLUTION ______________
SPECIAL REQUIREMENTS (Circle all that apply):
-Cold Air ____deg C -Flash Freezing
-Chemistry Lab -Cold Room
-On-site crystallizations -On-site Derivative Soaks
-Fluorescece detector
-Other _______________________
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