Crystal Screen cover slips

bill lodi at dino.qci.bioch.bcm.tmc.edu
Fri Jul 21 16:18:39 EST 1995


 In article <806346120-0-18554 at blue.weeg.uiowa.edu> sfredric at blue.weeg.uiowa.edu writes:
 >Thanks to all who responded to my previous query concerning glycerol and 
 >crystallization.
 >
 >And now. . . .I would appreciate hearing about efficient ways to siliconize 
 >coverslips.  My 1st few attempts, using Sigmacote, have generated spotty 
 >coverslips.  Thanks, Scott

 Tell you what... I'll trade you.  For siliconizing cover slips with
dichlorodimethylsilane, make a 5% v/v solution in toluene.  Have a
beaker of toluene ready for the rinse, DON'T use water at this stage, it
will make a gummy mess.  Rinse with ethanol, then ddH2O.  Remember di-
chlorodimethylsilane is nasty stuff.

 Also, this 5% solution can be diluted 1:100 with toluene to give a 0.05%
solution which can be used to briefly rinse x-ray capillaries (then 
rinse with ethanol), yielding a lightly siliconized cap that cleans up
easily (i.e. quick to get rid of excess m.l.) without giving crystal
slippage. (Your mileage may vary.)

  Now, how about a summary of the glycerol tips?

Thanks

W. E. Meador






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