Getting better xtals

Dave Schuller schuller at indigo2.biomol.uci.edu
Fri Jul 28 17:16:44 EST 1995


In article <3vb5rl$4c7 at moonbeam.aecom.yu.edu>, Puius <puius> writes:

|> As I up the pH and decrease [salt] I get fewer and bigger stars,
|> but no discrete xtals.  Is this a nucleation problem, i.e. should
|> I use compressed air to blow off dust?  Any other ideas?

. set up matrices to optimize each crystallization parameter separately,
 including but not limited to pH and [salt]. i have even had situations
where [buffer] made a difference. don't forget [protein].

. try different salts.

. try "additives", such as N-octyl glucoside or MPD in small amounts.

. try a larger drop size to slow crystal growth.

. try using your 'stars' for seeding.

. take a trip in the space shuttle.

. make sure your protein is really pure, and centrifuge it just before
setting up crystals to remove any precipitate which could increase
nucleation.

-- 
=======================================================================
                        Dave Schuller
                        University of California-Irvine
                        schuller at indigo2.biomol.uci.edu
                        modern man in a post-modern world




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