molecular replacement question
Y. W. Chen
ywchen at laue.biochem.ubc.ca
Thu Mar 30 14:54:03 EST 1995
I have a structure which has one molecule in the asymmetric unit of P6222. From homologous structures, I believe this monomer forms dimer related by a crystallographic 2-fold.
Having got no obvious solution for cross-rotation function using the monomer as a search model, in space group P6222, I would like to try using the dimer to search in P62. Since the rotation axis is fixed, I wonder if there is a way to restrict the cross-rotation search to be along a known axis. I use the CCP4 suite so I would appreciate your advises in response to using CCP4 programs, ie AMORE, ALMN, POLARRFN, and X-PLOR as well. I suspect I can use POLARRFN and search in the third polar angle (rotation along the axis). Please advise if there are other methods in doing this. Thanks in advance.
Yu Wai CHEN Department of Biochemistry
................................. & Molecular Biology
Faculty of Medicine
tel: (604)-822 5007 2146 Health Sciences Mall
fax: (604)-822 5227 University of British Columbia
email: ywchen at laue.biochem.ubc.ca Vancouver BC V6T 1Z3 CANADA
More information about the Xtal-log