CRYO PROTECTANT

[Joachim Seemann]

Hello there,

who know's useful cryo protectants other than
 PEG, glycol, glycerol, sucrose, MPD ?

My xtals tolerate glycerol but get destroyed on moving the loop to
the cold stream or freezing.
The precipitant ammonium sulfate doesn't allow sufficiently high con-
centrations of MPD or PEG. They will then phase separate.
As the 'propane technique' isn't yet established in our lab, I would
like to play around with protectants first.

What is your favorite method to take the loop into the cold stream?

Any suggestions are warmly appreciated.

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+           Joachim (Jo) Seemann                    +
+           Institut fuer org. Chemie und Biochemie +
+           Albertstrasse 21                        +
+           D-79104 Freiburg i.Br. (Germany)        +
+           Tel.: 0761/203-6061 FAX: 0761/203-5987  +
+ Email: seemann at bio5.chemie.uni-freiburg.de        +
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