seemann at bio5.chemie.uni-freiburg.de
Fri Nov 3 13:53:16 EST 1995
who know's useful cryo protectants other than
PEG, glycol, glycerol, sucrose, MPD ?
My xtals tolerate glycerol but get destroyed on moving the loop to
the cold stream or freezing.
The precipitant ammonium sulfate doesn't allow sufficiently high con-
centrations of MPD or PEG. They will then phase separate.
As the 'propane technique' isn't yet established in our lab, I would
like to play around with protectants first.
What is your favorite method to take the loop into the cold stream?
Any suggestions are warmly appreciated.
+ Joachim (Jo) Seemann +
+ Institut fuer org. Chemie und Biochemie +
+ Albertstrasse 21 +
+ D-79104 Freiburg i.Br. (Germany) +
+ Tel.: 0761/203-6061 FAX: 0761/203-5987 +
+ Email: seemann at bio5.chemie.uni-freiburg.de +
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