CRYO PROTECTANT
Hakon Hope
hhope at ucdavis.edu
Tue Nov 7 13:29:31 EST 1995
Response to Ana Gonzalez (gonzalez at esrf.fr)
The description of your experiments does not contain enough
detail to draw any good conclusions. I have a couple of
thoughts, though:
If your crystal was suspended in a thin solven film,
evaporation of water could be fast enough to change the
composition so that effectively the film was cryoprotected.
The normal behavior would be for ice to form from the mother
liquor, both in liq N2, liq propane, or in a cold stream.
Do you know that the ice formed on cooling in liq N2,
and not during the transfer to the diffractometer?
Hakon Hope
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