DLS Vs SEC

William Tivol tivol at news.wadsworth.org
Wed Nov 29 18:42:35 EST 1995


James Fethiere (James.Fethiere at BRI.NRC.CA) wrote:

: So which experiment should i believe, and do any of you have any suggestions
: to confirm these results.

Dear James,
	You could try a sedimentation equilibrium run on an ultracentrifuge
(the 230 nm absorbance of the backbone will give you increased sensitivity
if you need it).  See, e.g., Effect of Protein Concentration on the Molec-
ular Weight of Delta-5-3-Ketosteroid Isomerase by W.F. Tivol, E.D. Beckman
and W.F. Benisek, J. Biol. Chem. Vol. 250 (1975) 271-275.
				Yours,
				Bill Tivol




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