Protein expression in D2O

[Zophonias O. Jonsson]

Dear friends!

  We want to get large amounts of deuteriated protein by expression in
E.coli grown in D2O (for neutron scattering experiments).  Because of the
price of the substance we would be very thankful for any advice that could
save us experimentation.  A simple medline search revealed only one paper
on the subject.  If you have any experience, protocols or references that
could be of help we would be very happy.  Even educated guesses about what
to use as a carbon source for the bacteria would be helpful (as well as
other tips about other components of a suitable growth medium).

Sincerely

Zophonias

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Zophonias O. Jonsson
Institut fur Veterinarbiochemie               Tel: (41-1)-257-54-75
Universitat Zurich-Irchel                     Fax: (41-1)-362-05-01
Winterthurerstrasse 190
CH-8057 Zurich
Switzerland
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