Protein expression in D2O

numare at cnj.digex.net numare at cnj.digex.net
Tue Aug 6 18:09:39 EST 1996





In article <zjons-0608961029560001 at 130.60.120.10>, <zjons at vetbio.unizh.ch> 
writes:
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> From: zjons at vetbio.unizh.ch (Zophonias O. Jonsson)
> Newsgroups: bionet.xtallography,bionet.structural-nmr
> Subject: Protein expression in D2O
> Date: Tue, 06 Aug 1996 10:29:56 +0000
> Organization: Universitat Zurich-Irchel
> Lines: 23
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> 
08 August 1996, Tuesday, 1625 EDT

Dear Zophonias,

	Don't know about specifice techniques or lit. refs., however,
to save funds, why not setup your laboratory as a clearing house for spent
deuterium oxide?  Seems ashame that this expensive material is most often 
discarded after use as NMR solvent.  Simple filtration and distillation
would return it to usable form for your laboratory to use.  
	I imagine that if you offered to pay for shipping, you might very soon
have all the D2O that you will ever need. 

Good luck, 
Best regards,

Lawrence Byrnes,  NMR spectroscopist,
Numare Spectralab Incorporated,
email    numare at cnj.digex.net
www      http://www.cnj.digex.net/~numare/

> Dear friends!
> 
>   We want to get large amounts of deuteriated protein by expression in
> E.coli grown in D2O (for neutron scattering experiments).  Because of the
> price of the substance we would be very thankful for any advice that could
> save us experimentation.  A simple medline search revealed only one paper
> on the subject.  If you have any experience, protocols or references that
> could be of help we would be very happy.  Even educated guesses about what
> to use as a carbon source for the bacteria would be helpful (as well as
> other tips about other components of a suitable growth medium).
> 
> Sincerely
> 
> Zophonias
> 
> _____________________________________________________________________
> Zophonias O. Jonsson
> Institut fur Veterinarbiochemie               Tel: (41-1)-257-54-75
> Universitat Zurich-Irchel                     Fax: (41-1)-362-05-01
> Winterthurerstrasse 190
> CH-8057 Zurich
> Switzerland
> _____________________________________________________________________
> 






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