NADPH soaks

Christopher Walentas cdw1000 at mole.bio.cam.ac.uk
Tue Dec 3 17:02:20 EST 1996


Hi.

I've been trying to soak some crystals of a dehydrogenase with
NADPH but to no avail.  Does anyone out there have practical epxerience
with using NADPH (or any similar air-oxidisable coenzyme) and moreover
is willing to divulge the technique required to observe good density.
The enzyme binds it well, but my fear is that is it air oxidising.
Any and all help in this matter, even a reference, would be of great
help.

Thanks 

Tripp





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