soaking protein crystals with a colored compound

Peter R David david at lama.stanford.edu
Thu Feb 1 10:51:54 EST 1996


	Change of color of the crystals implies some type of binding,
but may also be an indication of protein denaturation.  There is
certainly no particular reason to believe that the color change is due
to specific binding.  

	As Balendra noted, you have to compute the difference fourier
to find specific binding.  Note that some specific binding does not
rule out extensive non-specific binding.  There are a number of well
known biochemical procedures to check the molar binding ratios of
compounds.

	Good luck,

	Peter David




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