Growth media for Selenomethionine modified proteins

Song Tan tan at mol.biol.ethz.ch
Fri Feb 16 02:36:56 EST 1996


In article <31232F2D.41C6 at xray.ki.ku.dk>, Michael Gajhede
<michael at xray.ki.ku.dk> wrote:

> I am very interested in references to papers with detailled description
> of optimal growth media for fermentation of cells producing recombinant
> proteins with methionines substituted to selenomethionines.
> Can anyone help ?

Check out:

Budisa, Steipe, Demange, Eckerskorn, Kellerman and Huber, High-level
biosynthetic substitution of methionine in proteins by its analogs
2-aminohexanoic acid, selenomethionine, telluromethionine and ethionine in
E. coli, European Journal of Biochemistry, 230, 788-796, 1995.  

Quite a nice paper with lots of details.  Should be just what you're
looking for.

-- 
Song Tan
Institute for Molecular Biology and Biophysics
ETH-Honggerberg (Swiss Federal Institute of Technology)
8093 Zurich, Switzerland
email:  tan at mol.bio.ethz.ch




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