Cryoprotectant for Xtals in (NH4)2SO4

luc moulinier moumou at titus.u-strasbg.fr
Tue Feb 20 12:09:17 EST 1996


>I have got Xtals in 2M Ammonium Sulphate, and cannot find a suitable
>cryaprotectant that they can survive in. Any suggestions? Has anyone had
>experience of freezing Xtals in this high a concentration of Ammonium
>Sulphate?
>
>--
>Alison Murray
>University of Bristol

We successfully recorded crystals which had been grown in 2.4M ammonium
sulfate and then stabilized in 4M ammonium sulfate , droping them in a 20 %
glycerol solution for 30 sec.  They were mounted in a loop.

Hope this help



Best regards

--------------------------------------------------------------------------------

Luc Moulinier
tel: (+33) 88 65 33 14
UPR 9004 de Biologie Structurale du CNRS
IGBMC
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