Cryoprotectant for Xtals in (NH4)2SO4

Tuomo Glumoff tglumoff at btk.utu.fi
Thu Feb 22 01:58:46 EST 1996


We had the following case:

Crystallization conditions: 1.56M AS + 100mM PIPES, pH 5.7

Cryo conditions: 40% (w/v) PEG-400 + 100mM PIPES, pH 5.7

Xal survives in the latter solution for 10 minutes, not more. We simply
soaked for 7 to 10 minutes and freezed. Works well.

Tuomo Glumoff
Centre for Biotechnology, Turku, Finland






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