Call for Synchrotron Beamtime Proposals

Robert M. Sweet SWEET at BNLARP.BNL.GOV
Fri Feb 23 11:20:34 EST 1996


     THIS IS A CALL FOR PROPOSALS FOR MACROMOLECULAR CRYSTALLOGRAPHY

         PROJECTS THAT REQUIRE THE USE OF SYNCHROTRON RADIATION.  

             >> Recent successes listed at the bottom <<

Two National Synchrotron Light Source beamlines are available under the
auspices of the Biology Department at Brookhaven National Laboratory. 
Information about these beamlines, X12-B and X12-C, is available on the
World-Wide Web: 

	http://bnlstb.bio.bnl.gov/                 Lists all facilities and 
                    |                                     Points to
                    V
	http://http://crim12b.nsls.bnl.gov/x12b.htm     Beamline X12-B

                   and

        http://lsx12e.nsls.bnl.gov/x12c/x12cinfo.html   Beamline X12-C

Both of these last two web sites point to the application materials, which
are common to the two beamlines. 

               Recent Successes
               ================

We have had recent success with unusually high resolution data: The Alpha-1
protein tetramer, a 440 atom structure, was solved entirely by
Shake-And-Bake, a direct-methods protocol used widely for small molecules.
The data were measured to 0.92-Ang. resolution. 

We have an Automatic Procedure for measurement of MAD data. Collection at
several wavelengths can be set up only once for sequential measurement.  To
assure that precisely the right wavelength is used, the x-ray absorption
spectrum of the heavy atom is scanned automatically just before the sweep
is started.  This coding of several sweeps makes straightforward the
measurement of Bijvoet pairs by the Friedel Flip method. 




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