how to cleave His-tag

[Gilles Truan]

V. Yee wrote:
> 
> We'd like to engineer a cleavage site in our His-tagged protein
> but cannot use a thrombin, fXa, or enterokinase site since these
> are calcium-requiring enzymes and we need to keep calcium from
> our protein.  Does anyone have a suggestion for a non-calcium
> dependent protease that we can use, and which has been successfully
> used by others (we are considering trypsin and chymotrypsin, but
> are likely to have cleavage at more than the one desired site)?
> 
> Many thanks!
	Life Technologies provides a vector containing a virus protease included in the Nterminus of the 
protein.. The name of the vector is pEX if I remember well. Anyway, you can find the kit on their web site 
probably.
-- 
Dr. Gilles TRUAN                         Phone # :(214) 648-4741                
Department of Biochemistry                 Fax # :(214) 648-8856                
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Tout va très bien, Madame la Marquise, tout va très bien, tout va très bien...