how to cleave His-tag
truan at utsw.swmed.edu
Tue Jul 16 14:29:51 EST 1996
V. Yee wrote:
> We'd like to engineer a cleavage site in our His-tagged protein
> but cannot use a thrombin, fXa, or enterokinase site since these
> are calcium-requiring enzymes and we need to keep calcium from
> our protein. Does anyone have a suggestion for a non-calcium
> dependent protease that we can use, and which has been successfully
> used by others (we are considering trypsin and chymotrypsin, but
> are likely to have cleavage at more than the one desired site)?
> Many thanks!
Life Technologies provides a vector containing a virus protease included in the Nterminus of the
protein.. The name of the vector is pEX if I remember well. Anyway, you can find the kit on their web site
Dr. Gilles TRUAN Phone # :(214) 648-4741
Department of Biochemistry Fax # :(214) 648-8856
UTSW Medical Center E-mail :truan at utsw.swmed.edu
5323 Harry Hines Blvd http ://pooh.swmed.edu/~truan
Dallas, TX, 75235-9038
Tout va très bien, Madame la Marquise, tout va très bien, tout va très bien...
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