Hi to all bug-people :
I wonder whether someone could give me some leads about how to express proteins
with Se-Met for MAD phasing. I read the review by Ms. Doublie and Charlie Carter but
that's it about. In particular, I'd like to know about suitable bug strains, new reviews and recipes, and
good and bad stories, for example, how transferable protocols are that already worked
in other E.Coli strains, and what about the oxidation issues.
Cheers,
BR
rupp1 at llnl.gov