Se-Met protein expression

Bernhard Rupp rupp1 at llnl.gov
Mon Mar 25 21:45:27 EST 1996


Hi to all bug-people :

I wonder whether someone could give me some leads about how to express proteins 
with Se-Met for MAD phasing. I read the review by Ms. Doublie and Charlie Carter but
that's it about.  In particular, I'd like to know about suitable bug strains, new reviews and recipes, and
good and bad stories, for example, how transferable protocols are that already worked 
in other E.Coli strains, and what about the oxidation issues.

Cheers, 

BR

rupp1 at llnl.gov




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