Se-Met protein expression

Satish Nair satish at a.chem.upenn.edu
Tue Mar 26 10:43:24 EST 1996


In article <4j7lo7$ij0 at lll-winken.llnl.gov> rupp1 at llnl.gov writes:
>Hi to all bug-people :
>
>I wonder whether someone could give me some leads about how to express proteins 
>with Se-Met for MAD phasing. I read the review by Ms. Doublie and Charlie Carter but
>that's it about.  In particular, I'd like to know about suitable bug strains, new reviews and recipes, and
>good and bad stories, for example, how transferable protocols are that already worked 
>in other E.Coli strains, and what about the oxidation issues.
>

Since you brought this up... I've been told that it's posssible to make
BL21(DE3) into a methionine auxotroph by spiking the growth media with
amino acids which inhibit methionine synthesis.  Wouldn't it then be possible
to incorporate Se-Met into BL21(DE3) expressed proteins or are there
other complications?

Just curious.

satish nair







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