rcbi at BEPC2.IHEP.AC.CN wrote:
>> Our research group study on the microgravity effects on protein
>crystallization. I have finished high resolution space- and earth-grown
>crystals' structure refinement of an protein, and I am now trying to
>compare their structure.
>> I am very interested in comparing their solvent structure, but I
>do not think that I have good and effective ways to do this.
>Jishen Pan
In order to gain any statistically significant insight into differences
in water structure between earth and space grown crystals, I think you
would need (many) more than one separate refinement of each. There can
even be significant differences between solvent structures for crystals
grown under identical condition (even from the same pot). An early
mention of this was in crambin by Hakon Hope in 1988 (Acta Cryst. B44,
22-26). Since then, we have had similar experiences with a number of
very high resolution protein refinements.
Without a rather large sampling of earth and space grown crystal
refinements, it would be near impossible to attribute differences in
solvent structure to the effects of microgravity.
Sean Parkin
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| Dr. Sean Parkin | phone (510) 423-5427 |
| Biology and Biotechnology Research Program | fax (510) 422-2282 |
| Lawrence Livermore National Laboratory | home (209) 833-0713 |
| 7000 East Avenue | |
| Livermore, CA. 94550 | sp at oedipus.llnl.gov |
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