Hi, everyone,
I am using CCP4.3 to determine heavy atom positions from anomalous
difference patterson map of Se-Met protein. There should be
sixteen Se sites. The space group is P21.
I am following the sample command file in
$CDOC/rsps.doc to run Harker and polarscan search in P21. The
Harker section has eight peaks which are higher than 4*sigma. I
then ran polarscan search fixing either one of the eight positions
which corresponds to one of the eight harker section peaks. The problem is
that the search results do not match each other, which is to say that if
I fix position 1, I might find position 2 but when I fix position 2
the position 1 resulted from polarscan will have a different y.
Does anyone have any idea how to interprete the results and
find the heavy atom sites or do you have a command file that works for P21?
Or is there any other suggestion to determine the sixteen
heavy atom sites?
Any help will be highly appreciated.
Joyce
