Enzyme activity in crystals

Ditlev Brodersen ditlev at kemi.aau.dk
Wed Oct 9 03:53:03 EST 1996


>Could anyone please provide me with some references where enzyme or
>other biology activity has been demonstrated in a protein crystal?
>
>Email is fine--no need to clutter up the newsgroup with this oldie but
>goodie sort of information.
>
>Thanks.
>
>TG
>
>--
>_____________________________________________________________
>Terry M. Gray, Ph.D. Department of Chemistry and Biochemistry
>Calvin College       3201 Burton SE   Grand Rapids, MI  40546
>Office: (616) 957-7187                    FAX: (616) 957-6501
>Email: grayt at calvin.edu          http://www.calvin.edu/~grayt
>
>*This mission critical message was written on a Macintosh with Eudora
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>
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Hi Terry,

  the group of G. L. Rossi at University of Parma, Italy, has worked for a
great many years on demonstrating and measuring catalytic activity of
protein crystals, e.g. the binding of oxygen by hemoglobin.
  I worked in their lab two years ago, where we showed by polarized
absorbance spectroscopy that crystals of ternary complexes of methylamine
dehydrogenase (MADH), amicyanin, and cytochrome c551i do indeed work as
electron transfer proteins also in the crystalline state.

A. Merli, D. E. Brodersen, B. Morini, Z. Chen, R. C. E. Durley, F. S.
Mathews, V. L. Davidson, and G. L. Rossi, J. Biol. Chem (1996), 271(16),
9177-9180

-- Ditlev

-------------------------------------------------------------------
Ditlev Brodersen                   Phone: +45 8942 3871
Dept. of Struct. and Mol. Biology  Fax:   +45 8619 6199
c/o Dept. of Chemistry             Email: ditlev at kemi.aau.dk
Langelandsgade 140                        ditlev at beauty.kemi.aau.dk
DK-8000 Aarhus C
Denmark

WWW:http://kaktus.kemi.aau.dk/~ditlev






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