B correlation with TNT

Poul Nissen nissen at KEMI.AAU.DK
Tue Sep 17 09:58:49 EST 1996


I have refined a protein-tRNA complex with TNT with the use of very
naive
restraints on the B-factors in tRNA:
identical B-factors among all bonded atoms,
a sigma of 10 for atoms of bases
a sigma of 15 for atoms of riboses
and a sigma of 20 for atoms in the phosphate-linkage
These restraints are clearly not ideal, but they improved
the free R-factor with about 1% right away upon their introduction
(from 31% to 30% with 25 - 2.7 A data)

Poul Nissen




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