HI Arioch,
In our lab, the native and hig-tagged proteins are crystallized
under very similar conditions. And the his-tagged protein
seems to crystallize a bit more easily, at least in several
other forms and conditions. Well, this depends of the
nature of the protein and the packing in the crystal lattice.
Another protein in the lab, without the tagged-histidines,
never crystallizes.
Hope this help.
Ping Kongsaeree
zb00041 at uabdpo.dpo.uab.edu wrote:
>> hey all,
> ...i'm currently working with a hist-tagged protein (using novagen's
> pET vecor system...) and would like to solve the crystal structure.
> does anybody know of any past refernces of his-tagged protein s that
> have been crystallized in the past WITH the his-tag on?? any help here
> would be appreciated. also any previous refernces to GST-fusion protein
> crystallizations with the GST tag on as well would also be
> appreciated...please email to siyer at bmg.bhs.uab.edu . thx much...
> --
> Arioch
>siyer at bmg.bhs.uab.edu> Graduate Student (and part time Lord of Chaos)
> Dept. of Biochemistry and Molecular Genetics
> University of Alabama at Birmingham...
--
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Palangpon Kongsaeree Phone: (607)255-6145
Chemistry Department Fax: (607)255-1253
Cornell University,
Ithaca, NY 14853 E-mail : pk11 at cornell.edu
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