molecular replacement...

sai at uab.edu sai at uab.edu
Mon Apr 14 19:30:29 EST 1997


hey all,
           i'm planning to crsytallize and solve the structure of the
protein that i'm working on.  there are no homologous structures that
hve been reported for my protein.  but i would like to take advantage of
the fact that i have a thioredoxin fusion protein and the structure of
the thioredoxin protein has been determined.  my protein is 52.5 kDa and
the thioredoxin is ~11.6 kDa, making the total mass of my fusion protein
at around 64.1 kDa.  I would like to know how much homology i need to be
able to reliably use the solved structure of the thioredoxin (comprising
abour 18% of the fusion protein) as molecular replacement to solve the
structure of my protein???  i want to know this in hopes of avoiding
doing heavy atom derivatives (or is that just too inevitable)...please
email to sai at uab.edu .  thanks much...


--
sai 
graduate student
dept. of biochemistry and molecular genetics
univ. of alabama at birmingham...




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