Processing With Denzo
ash at bio.cornell.edu
Thu Jan 9 20:25:53 EST 1997
The problems you observe are almost certainly related to the use
of cryocooling and are probably indicative of some major changes
upon freezing. Other relevant questions are:
Did you observe any increase in resolution for the low
How well did the crystals freeze - was
there any dramatic increase in mosaicity?
What is the Matthews number / solvent content for your protein?
What is the morphology of the crystal and how does this relate
to the cell axes and the changes you observe?
Have you tried merging or scaling room temperature and low
temperature data and are they isomorphous (Both in Scalepack and / or
with SCALEIT - CCP4)?
How are the Rmerge statistics for the low temperature
synchrotron data compared with the room temperature?
Do you have the structure of this protein?
Hope you find the answers,
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