Mercury Soak for Lysozyme.

islam islam at icrf.icnet.uk
Fri Jan 17 07:14:25 EST 1997


> I was wondering if anyone knows of the best mercury salt to use to obtain a
> single sited mercury derivative of Hen Egg White Lysozyme in sodium chloride?
> The original studies used PCMBS (para-chloromercuribenzene sulphonate) but gave
> no specific details.


Hello,

Below are some details of 7 experimental conditions used for
Lysozyme-HG derivative. This is part of our HEAVY ATOM DATABANK
which is being setup (not announced yet) at

        http://bonsai.lif.icnet.uk/bmm/software.html

Hope it helps. If you have further queries (e.g. references)
please email me.

The data below may seem cryptic but the essential keywords
are:
HCOD - heavy atom derivative
HCON - hcod concentration   
PPPT - percipitant
PCON - pppt concentration
BUFF - buffer
BCON - buffer concentration
ADDT - addictive
ACON - addt concentration
PHPH - pH   

REFS 111.00
BCOD 1LYZ@
PNAM LYSOZYME (HEN EGG-WHITE W2)
PNAM
HCOD 2-HYDROXYMERCURI-TOLULEN-4-SULPHONIC ACID (HMTS)
HCON   -1.0
PPPT SODIUM CHLORIDE
PPPT
PCON    1.00000
BUFF
BUFF
BCON    0.00000
ADDT
ADDT
ACON    0.00000
SOAK    0.00000
SPEC
SPEC
PHPH    4.70000
TMAU
BIND   1A39.0/ARG 68 +46:
BIND   1B
BIND   2A9.0/
BIND   2B
ENDC  111
 
REFS 111.03
BCOD 1LYZ@
PNAM LYSOZYME (HEN EGG-WHITE W2)
PNAM
HCOD PARA CHLOROMERCURI BENZENE SULPHONATE (PCMBS)
HCON   -1.0
PPPT SODIUM CHLORIDE
PPPT
PCON    0.85000
BUFF
BUFF
BCON    0.00000
ADDT
ADDT
ACON    0.00000
SOAK    0.00000
SPEC
SPEC
PHPH    4.70000
TMAU
BIND   1A?/ARG 68 +46:
BIND   1B
ENDC  111
  
REFS 111.08
BCOD 1LYZ@
PNAM LYSOZYME (HEN EGG-WHITE W2)
PNAM
HCOD POTASSIUM TETRAIDO MERCURATE (II)
HCON   -1.0
PPPT SODIUM CHLORIDE
PPPT
PCON    0.85000
BUFF
BUFF
BCON    0.00000
ADDT
ADDT
ACON    0.00000
SOAK    0.00000
SPEC
SPEC
PHPH    4.70000
TMAU
BIND   1A?/ARG 13:ARG 13:
BIND   1B
BIND   2A?/
BIND   2B
BIND   3A?/
BIND   3B
ENDC  111
 
REFS 127.00
BCOD 1LZ1@
PNAM LYSOZYME (HUMAN)
PNAM
HCOD MERCURY (II) ACETATE
HCON   50.00000
PPPT SODIUM CHLORIDE
PPPT
PCON    3.00000
BUFF SODIUM ACETATE
BUFF
BCON    0.02000
ADDT
ADDT
ACON    0.00000
SOAK    0.00000
SPEC
SPEC
PHPH    4.50000
TMAU
BIND   1A49.0/
BIND   1B
BIND   2A29.0/
BIND   2B
ENDC  127
 
REFS 171.00
BCOD 0LZ6
PNAM LYSOZYME (STREPTOMYCES ERYTHRAEUS)
PNAM
HCOD MERCURIC (II) CHLORIDE
HCON   10.00000
PPPT AMMONIUM SULPHATE
PPPT
PCON   -8.,20.,
BUFF
BUFF
BCON    0.00000
ADDT
ADDT
ACON    0.00000
SOAK    0.00000
SPEC SOAK VARYING PERIODS
SPEC
PHPH    0.00000
TMAU
BIND   1A67.0/
BIND   1B
BIND   2A27.0/
BIND   2B
BIND   3A12.0/
BIND   3B
BIND   4A15.0/
BIND   4B
BIND   5A6.0/
BIND   5B
BIND   6A14.0/
BIND   6B
BIND   7A14.0/
BIND   7B
BIND   8A11.0/
BIND   8B
BIND   9A14.0/
BIND   9B
BIND   10A8.0/
BIND   10B
BIND   11A12.0/
BIND   11B
BIND   12A5.0/
BIND   12B
BIND   13A12.0/
BIND   13B
BIND   14A7.0/
BIND   14B
ENDC  171
 
REFS 250.00
BCOD 2LZH@
PNAM LYSOZYME (HEN EGG WHITE, ORTHORHOMBIC)
PNAM
HCOD  MERCURY (II) ACETATE
HCON   20.00000
PPPT SODIUM CHLORIDE
PPPT
PCON    0.00000
BUFF
BUFF
BCON    0.00000
ADDT
ADDT
ACON    0.00000
SOAK    0.00000
SPEC
SPEC
PHPH    7.50000
TMAU
BIND   1A0.386/
BIND   1B
BIND   2A0.414/
BIND   2B
ENDC  250
 
REFS 250.00
BCOD 2LZH@
PNAM LYSOZYME (HEN EGG WHITE, ORTHORHOMBIC)
PNAM
HCOD  MERCURY (II) ACETATE
HCON   20.00000
PPPT SODIUM CHLORIDE
PPPT
PCON    0.00000
BUFF
BUFF
BCON    0.00000
ADDT
ADDT
ACON    0.00000
SOAK    0.00000
SPEC
SPEC
PHPH    7.50000
TMAU
BIND   1A0.386/
BIND   1B
BIND   2A0.414/
BIND   2B
ENDC  250

____________________________________________
Suhail A Islam
Biomolecular Modelling Laboratory
Imperial Cancer Research Fund, P.O. Box 123
44 Lincoln's Inn Fields, London WC2A 3PX   
Tel: (0171) 269 3380, Fax: (0171) 269 3479
email: islam at icrf.icnet.uk
http://www.icnet.uk/bmm/  
____________________________________________




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