Enzymatic removal of sugars

CS Raman raman at HEMEBASE.BIO.UCI.EDU
Mon Jun 30 11:20:00 EST 1997


Gitay:

{*} I am looking for information regarding the enzymatic removal of sugars
{*} from glycosilation sites of proteins in order to facilitate
{*} crystallization.

You may want to take a look at the following article for exactly
what you are searching for:

Grueninger-Leitch F; D'Arcy A; D'Arcy B; Chene C.
Deglycosylation of proteins for crystallization using recombinant fusion
protein glycosidases.
Protein Science, 1996 Dec, 5(12):2617-22.

Cheers
-raman
___________________________________________________________________
C.S.Raman                    Tel: (714) 824-4322
University of California     Fax: (714) 824-8540 
Dept. MB & B                 email: raman at indigo1.bio.uci.edu
3205 Bio Sci II
Irvine, CA 92697-3900
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