IUBio

Problems with thin protein crystals

Mahieddine Ladjadj tivol at news.wadsworth.org
Thu Mar 6 11:52:31 EST 1997


: Steffen Graether wrote:
: > 
: > Hello,
: > 
: >         We have been able to get thin (>0.05 mm) crystals that grow
: > relatively well in the other two dimensions, but this results in weak
: > diffraction patterns (~5000 s exposure per frame).  Coventional seeding
: > techniques fail. Could anyone provide suggestions and ideas on how I could
: > grow thicker crystals?

Dear Steffen,
	In case you are unable to get thicker crystals, you may want to
consider doing electron crystallography.  For that you will need thinner
crystals (a few tens of nm) in order to minimize multiple scattering.
If you are interested, please let me know.
				Yours,
				Bill Tivol




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