>Hi folks,
>>any suggestions on where to get hold of some Nicron
>wire for making cryo-loops, preferable somewhere in
>Europe? I need the extra stability for a long needle-
>shaped crystal.
>>CU
> /Arnold
>>+++++++++++++++++++++++++++++++++++++++++++
>Arnold Andersson (Ph.D)
>Max-Planck Inst. for Medical Research 69120 Heidelberg, Germany
>Tel: +49-6221-486286 E-mail: arnold at otto.mpimf-heidelberg.mpg.de
For long needles, you can try the "old fashion" spatula. We were
successful using these with long needles, since you can gently
pick up your crystals with only about 1/3 of the crystal on
the spatula itself and the rest sticking out. This way you also get
very little absorption from mother liquor since there would only
be a thin film around the crystal itself. We of course center
the part of the crystal that is away from the spatula in the beam.
--Leemor
*******************************************************************
Leemor Joshua-Tor, Ph.D.
Assistant Investigator
Keck Structural Biology
Cold Spring Harbor Laboratory Tel. (516) 367 8821
1 Bungtown Road Fax (516) 367 8873
Cold Spring Harbor, NY 11724 e-mail: leemor at cshl.org
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