non-pro cis peptide bond

CS Raman raman at HEMEBASE.BIO.UCI.EDU
Wed Apr 8 07:57:34 EST 1998


Thomas:

May be not in the native form, but take a look at the following
article from Franz Schmid's group at Bayreuth.

Odefey C; Mayr LM; Schmid FX.
Non-prolyl cis-trans peptide bond isomerization as a rate-determining
step in protein unfolding and refolding.
Journal of Molecular Biology, 1995 Jan 6, 245(1):69-78.

In wild-type ribonuclease T1 the peptide bond between Tyr38 and Pro39
is in the cis conformation. When Pro39 is replaced by an alanine this cis
conformation is retained, and a non-prolyl cis Tyr38-Ala39 peptide bond is
generated.


Cheers
-raman

{*} RNAse T1 does not contain non X-Pro cis-peptide bonds.
{*} It does contain a number of X-Pro cis-peptide bonds. 
{*} All legume lectins contain an Ala-Asp cis-peptide bond. 
{*} Arcelin contains an Ala-Tyr cis-peptide bond. Concanavalin B
{*} also has one. 
{*} 
{*} Cheers,
{*} ------------------------------------------
{*} Thomas Hamelryck, thamelry at vub.ac.be
{*} Laboratorium voor Ultrastructuur, VIB-VUB
{*} Paardenstraat 65, B-1640 St-Genesius-Rode, Belgium
{*} "Sunday morning I'm waking up
{*}  Can't even focus on a coffee cup
{*}  Don't even know whose bed I'm in
{*}  Where do I start, where do I begin?"
{*} 
{*} 

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C.S.Raman                    Tel: (714) 824-4322
University of California     Fax: (714) 824-3280
Dept. MB & B, SH530          email: craman at uci.edu
3205 Bio Sci II
Irvine, CA 92697-3900
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