SeMet in Pichia Pastoris

Pawel Grochulski pawel at bch.umontreal.ca
Tue Dec 1 17:31:50 EST 1998


Hi Everyone,
I wonder if anybody has tried to express selenometionyl protein in
either Pichia pastoris or in SF9 insect cells? There are only two
reports
in the literature by Chen WY, et al. (1991), as far as the SF9 cells are

concerned. They claim that they got more than 84% of methionine residues

substituted by selenomethionine.

I would appreciate any hints and/or comments.

Pawel Grochulski

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BioMep, Université de Montréal,     Phone:  (514) 343-6111 x3828
Department of Biochemistry, D353,   Fax:    (514) 343-7018
2900 Edouard-Montpetit,             E-mail: Pawel at BCH.UMontreal.CA
Montreal, QC, H3T 1J4, CANADA              gpawel at yahoo.com
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