John Luz wrote:
> Hi Folks--Is there anyplace out there that I can buy a methione minus
> media for growing up bugs for selMET protein expression? Thanks
> much.--John
I assume you are trying to grow E. coli. I don't know of any
commercially available media mix for this, but Venki Ramakrishnan has
developed a protocol for expressing SeMet proteins very reliably. We've
used his protocol at least a dozen times now for various things, and it
works beautifully. Point your browser towards:
http://snowbird.med.utah.edu/~ramak/madms/segrowth.html
--
------------------------------------------------------------------------
Felix F. Vajdos (801)585-3921
University of Utah felix at alta.med.utah.edu
Dept. of Biochemistry
50 North Medical Drive
Salt Lake City, UT 84132
------------------------------------------------------------------------
"Truth is the child of time, not of authority. Our ignorance is
infinite, let's whittle away just one cubic millimeter. Why should
we still want to be so clever when at long last we have a chance of
being a little less stupid?"-----Bertolt Brecht's Life of Galileo
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