Daniel Lim wrote:
>> I'm currently trying to improve on a crystallization condition, for which
> crystals form very quickly (within 10 to 20 minutes).
>snip
> Does anyone have any suggestions about reducing the tendency for these
> kinds of clusters to form?
>> I have tried all of Hampton's additive and detergent screens,
> crystallization in agarose gel, increasing viscosity with carbohydrate or
> glycerol, playing with pH, temperature, protein concentration, salt
> concentration and buffer concentration.
>> Thanks in advance!
>> Dan
>dan at crystal.biochem.queensu.ca
In my case seeding helps a lot to get bigger Crystalls. I have the same
Problem and solved it by fetching one fine plate out of the drop with an
small nylon-loop (best made from a single tooth silk fibre) fixed with
super-glue on the top of a cappilary and put this small crystal or a
single needle from a cluster into a fresh Protein solution.
Growing big of this preceeding crystal should be kinetic favored over
the growing of new crystalls at low protein concentration.
Even micro-seeding with a lot of broken crystall fragments should lead
to bigger single-crystalls.
Good luck and fast growing crystalls , Marco
P.S. sorry for my bad english
--
Marco Bocola Inst. f. pharmaz. Chemistry
AG Prof. Klebe
Tel: +49-6421-28-5071 Marbacher Weg 6
Fax: -8994 D-35032 Marburg
e-mail:bocola at mailer.uni-marburg.de
:-( There are no problems, just solutions !? :-)
