Purifying halogenated DNA?
ng at mbi.ucla.edu
Tue Oct 20 17:00:01 EST 1998
Hi. I am having some problems purifying bromo and iodo DNA oligomers
(10 and 12 mers). During purification by anion exchange in 7M urea with
I usually see 2-3 significant peaks which overlap. I don't see these
peaks when purifying non-modified DNA. What are the components of these
peaks? And what is a good way to identify the correct peak? Thanks!
Ho Leung Ng
holeung at ucla.edu
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