maximum size

[Harry Powell]

> Hello,
> I working on the crystallization of Enniatinsynthetase, a 350 kDa enzyme,
> consisting of one polypeptid chain. I would like to know if a protein of=
=20
> comparable size has been crystallized yet. If yes, did it have more than=
=20
> one polypeptid chain?
> Thanks for your help,
> Christine

Bovine F1-ATPase, 370,000 Daltons, 5 polypeptide chains (Abrahams, J.P.,
Leslie, A.G.W., Lutter, R. & Walker, J.E. The structure of F1-ATPase from
bovine heart mitochondria determined at 2.85=C5 resolution. Nature 370,
621-628.).=20

Harry
--=20
Dr Harry Powell, MRC Laboratory of Molecular Biology,
MRC Centre, Hills Road, Cambridge, CB2 2QH