xengen-spots

[Krishnan, Raman]

Dear fellow xtallographers
thanx for all the suggestions. Thanx specially to Prof. Greg Petsko for
taking his time 
and replying. The crystals have stopped dissolving after a avoided the
mother liquor/storage solution plugs...
Iam using a X1000 siemens detector (sigh!!) to collect data. The Spots
routine
ppicks up a lot of spots but merges them to very less number of spots (> 5)
per 
frame. In addition the programme throws away too many spots as moving on the

second pass (Frambo) . everyting was fine till last week. My crystals look
the same!!
Is there anything wrong with the detector/software?
I work with smae crystals agaian and again (thrombin/trypsin). The indexing 
consequently is amess and the R merge goes to hell!!!
krishnan

Raman Krishnan
Research Scientist
Biocryst
2190, Parkway Lake Drive
Birmingham
AL 35244
off. 205-403-4677
home. 205-733-0981
EMail: RKrishnan at BIOCRYST.com
FAX: +1 205 444-4640
http://www.biocryst.com/


Have a nice day!!