Crystallization of His-tagged proteins

NA u23s at hotmail.com
Mon Apr 24 15:30:19 EST 2000


Hi,
assuming that the biochemical characteristics of a given His-tagged
protein are very similar to the wild-type enzyme, is there any general
reason not to try and (attempt to) crystallize the His-tagged protein?
In other words, does an N-terminal His-tag (in general) interfere with
crystallization, or not? Are there any reviews out there that discuss
the issue?
Thanks in advance,
Uli.









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