Spherulites....

[Mike Hanlon]

Spherulites can be a good sign - so try setting up an optimisation
screen. Varying the PEG concentration by a few % and the pH by 0.1steps
around the original conditions. Even a slight change of conditions can
make the difference between xtal and no xtal

Good luck

Debashis Mukhopakhyay wrote:

>
>
> Hi all,
>
> I'm looking for some suggestions regarding the following problem.
>
> After prolonged trial I got some spherulites for a protein I'm
>
> trying to crystallize, the conditions being 25-30% PEG300 in Citrate
>
> (pH 5.5). They are solid (crystal-like) to touch and also takes the
>
> Izit-dye (Hampton). I'm looking for ideas as to what to do next to
>
> get a nice diffracting crystal.
>
> Sharing of any experience in the matter would be highly appreciated.
>
> Thanks,
>
> Deb
> [32°51'47.120''North;117°13'55.495''West]
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> "Mind is like a parachute, if you don't open it, it won't work"
> ===============================================================
> ***********************************************************
>                  Debashis Mukhopadhyay, Research Associate
>       /\_/\      Dept. of Molecular & Experimental Medicine
>  /\  / o o \     Division of Cellular Biology
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>
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