Large Structures Meeting - Asilomar - April, 2002

[DAVID DEROSIER]

The Biophysical Discussions
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Frontiers in Structural Cell Biology:
Determining the structures of large subcellular machines
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Meeting Dates: April 20-22, 2002.

Meeting Site: Asilomar, California.  This is a spectacular site.
You can see it at http://www.asilomarcenter.com/.
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The biophysical discussions are set up so that the discussion time is
greater than the times allotted for presentations, which will be
available
on the web prior to the meeting.  The format is unusual, but highly
successful in the past.

To apply send a letter of intent to discussions at biophysics.org by
November
15, 2001. Space is limited.
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Organizing committee: Axel Brunger (Stanford University), David DeRosier

(Brandeis University), Steve Harrison (Harvard University), and Eva
Nogales
(University of California at Berkeley).

The Program  (Saturday, April 20 at 9 am to Monday April 22 at 12 noon)

Session I.   The state of structural biology of large structures.
            Moderator Helen Saibil
              Richard Henderson
                        The power of electron cryomicroscopy.
              Joachim Frank
                        The Ribosome -- a molecular machine in motion
              Jamie Cate
                        Biochemical basis for x-ray crystallography of
the
                         ribosome

Session II.   Extending x-ray crystallography to ever larger structures
            Moderator Keith Hodgson
              Andy Thompson
                        Can we routinely collect useful data from
                         micro-crystals?
  Janos Hajdu
            Future X-ray sources (tentative)
              Randy Reed
                        The phase problem: does size matter?

Session III.   New ways to obtain large complexes for structural studies

            Moderator Axel Brunger
            Don Wiley
                        Stabilizing multi-component biological complexes
for
                         structural studies by protein engineering,
                        expression, and refolding - AND -
                        avoiding artifacts
            TBA
                        Expression and Co-expression of components
                        (tentative title)


Session IV.   What does the future hold for electron cryomicroscopy?
            Moderator Bob Glaeser
            Niko Grigorieff
                       Single particles always fit the mold
            Ken Downing
                       The hybrid approach to electron crystallography
            Wolfgang Baumeister
                        Electron Tomography: Towards visualizing
                         macromolecular assemblies
                        inside cells
            Ed Egelman
                        Polymorphism, can we detect it? Can we use it?
Can
                        we control it?
                        Examples from actin and nucleoprotein complexes.

Session V.   Can hybrid methods provide credible atomic models?
            Moderator Eva Nogales
            Niels Volkmann
                        Atomic model of the cell: docking in a
tomographic
                        environment
            Willy Wriggers
                        Reconciling Shape with Structure: Morphometric
                        Strategies for Multi-Resolution Flexing