protein expression

Chris Upton chrisupton at mac.com
Mon Apr 15 09:00:49 EST 2002


Hello,

 has anyone here used the Gateway cloning - expression system for
producing high levels of proteins?  Any favorite vectors, kit or
homemade?

We're going to test 50+ genes and want to simplify cloning.  I've been
recommended the TOPO-PCR cloning for creating the entry vector -
comments?

many thanks,
  Chris Upton





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